heliX +
Bukana ea Mosebelisi
ADAPTER STRAND PAKAKE
ka dae e khubelu Ra
Dynamic Biosensors GmbH & Inc.
ASP-1-Ra v5.1
Likarolo tsa Bohlokoa
- Adapter strand 1 - Ra le Adapter strand 2 - Ra - lfs (ligand free strand) bakeng sa ts'ebetso ea heliX® Adapter Chip ho Spot 1 le Spot 2, ka ho latellana.
- E lumellana le heliX® Adapter Chip.
- E kenyelletsa likhoele tsa Adapter bakeng sa lintlafatso tse 50.
- E loketse MIX&RUN sample litokisetso.
- Likhoele tsa Adapter 1 le 2 li na le dae e khubelu ea hydrophilic (Ra) e nang le tefiso e le 'ngoe e ntle.
heliX® Adapter Chip Overview
Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA.
Tlhaloso ea Sehlahisoa
Nomoro ea Taelo: ASP-1-Ra
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo
| Lintho tse bonahalang | Cap | Ho tsepamisa maikutlo | Chelete | Buffer | Polokelo |
| Adapter strand 1 - Ra | Ntsho | 400 nM | 5 x 100µL | TE40 [1] | -20°C |
| Adapter strand 2 - Ra - lfs | Bosoeu | 200/250 nM | 5 x 200µL | TE40 [1] | -20°C |
Bakeng sa tšebeliso ea lipatlisiso feela.
Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho label.
Ho qoba linako tse ngata tsa ho qhibiliha ha leqhoa ka kopo, aliquot the nanolever.
Tokisetso | TŠOAKA&RUTHA
In-solution hybridization ea adaptara le likhoele tsa ligand:
- Kopanya Adapter strand 1 - Ra (400 nM) le conjugated Ligand strand (500 nM) ka karolelano ea 1: 1 (v / v).
- Eketsa tharollo ea mohato oa 1 ho RT ka 600 rpm bakeng sa metsotso e 30 ho etsa bonnete ba ho kopanya ka ho feletseng.
- Kopanya tharollo ea mohato oa 2 le Adapter strand 2 - Ra - lfs (200 nM) ka karolelano ea 1: 1 (v / v). Tharollo e se e loketse ho sebelisoa bakeng sa ts'ebetso ea biochip.
Ho tsitsa ha tharollo ho amana le botsitso ba molek'hule ea ligand.
Lethathamo la 2. Lintlha tse ling bakeng sa ts'ebetso ea sebaka sa 1 le sebaka sa 2 sa litšupiso.
| Lintho tse bonahalang | Ho tsepamisa maikutlo | Buffer | Related Product Name | Odara No |
| Ligand strand e jereng ligand e kopaneng | 500 nM | PE40 [2] | heliX® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Bophahamo bo hlokahalang bakeng sa lits'ebetso tse 3: 100 µL e nang le khatello ea ho qetela ea 100 nM.
| Vial 1 | Vial 2 | |
| Adapter strand 1 – Ra (400 nM) | Khoele e kopaneng ea Ligand (500 nM) | Adapter strand 2 – Ra – lfs (200/250 nM) |
| 25µL | 25µL | 50µL |
Kamora nako ea ho elisa, kopanya vial 1 le vial 2 ho fumana 100 µL ea tharollo ea DNA e seng e loketse ho sebelisoa.
Ikopanye
| Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Jeremane |
Litlhaloso tsa likarolo tsa Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 Woburn, MA 01801 usa |
Lintlha tsa Odara order@dynamic-biosensors.com
Tšehetso ea tekheniki support@dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Litokelo tsohle li sirelelitsoe.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
[2] Haeba protheine e sa tsitsa ho PE40 (TE40, HE40),
ka kopo, hlahloba ts'ebetso ea "buffer" le letlapa la ts'ebetso ea switchSENSE®.
ASP-1-Ra v5.1
www.dynamic-biosensors.com
Litokomane / Lisebelisoa
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dynamic BIOSENSORS ASP-1-Ra Adapter Strand Package [pdf] Bukana ea Mosebelisi ASP-1-Ra, ASP-1-Ra v5.1, ASP-1-Ra Adapter Strand Package, ASP-1-Ra, Adapter Strand Package, Strand Package |
