BIOSENSORS heliX e matla hammoho le Adapter Strand 1

Litlhaloso:

• Lebitso la Sehlahisoa: heliX +
• Mohlala: ADAPTER STRAND 1 e nang le dae e khubelu Ra
• Moetsi: Dynamic Biosensors GmbH & Inc.
• Nomoro ea Taelo: AS-1-Ra
• Maikutlo: 400 nM
• Storage: Black cap, Buffer TE40
• Bakeng sa tšebeliso ea lipatlisiso feela

Tlhaloso ea Sehlahisoa:

HeliX+ ADAPTER STRAND 1 e entsoe ka dae e khubelu ea Ra 'me e na le matheba a 2 a nang le tatellano ea 2 e fapaneng ea li-ankora bakeng sa aterese ea DNA-encoded. E tla ka bongata ba 400 nM mme e etselitsoe merero ea lipatlisiso.

Litaelo tsa Tšebeliso:
Tokisetso | MIX&RUN:

1. Kopanya Adapter strand 1 - Ra (400 nM) le conjugated Ligand strand (500 nM) ka karolelano ea 1: 1 (v / v).
2. Eketsa tharollo ho tloha mohato oa 1 mocheso oa kamore ka 600 rpm bakeng sa metsotso e 30 ho etsa bonnete ba ho kopanya ka ho feletseng.
3. Kopanya tharollo ho tloha mohato oa 2 le Adapter strand 2 - Ra - lfs (200 nM) ka karolelano ea 1: 1 (v / v).
4. Tharollo e se e loketse ho sebelisoa bakeng sa ts'ebetso ea biochip.

Tlhokomeliso: Ho tsitsa ha tharollo ho itšetlehile ka botsitso ba molek'hule ea ligand.

LBH:

1. Q: Bophelo ba shelofo ea heliX+ ADAPTER STRAND 1 ke bofe?
   A: Sehlahisoa se na le nako e lekanyelitsoeng ea bophelo, ka kopo sheba letsatsi la ho felloa ke nako ho label.
2. P: Na heliX+ ADAPTER STRAND 1 e ka hoamisoa?
   A: Ho qoba linako tse ngata tsa ho qhibiliha ha leqhoa, ho kgothaletswa hore o fane ka nanolever.

heliX+
Bukana ea Mosebelisi

ADAPTER STRAND 1
ka dae e khubelu Rb
Dynamic Biosensors GmbH & Inc.
AS-1-Rb v5.1

Likarolo tsa Bohlokoa

•  Adapter strand 1 - Rb bakeng sa ts'ebetso ea heliX® Adapter Chip Spot 1.
• E lumellana le heliX® Adapter Chip.
• E kenyelletsa likhoele tsa Adapter bakeng sa lintlafatso tse 50.
• E loketse MIX&RUN sample litokisetso.
• Adapter strand 1 e na le dae e khubelu ea hydrophilic (Rb) e nang le tefiso e sa nke lehlakore.

heliX® Adapter Chip Overview
Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA.

Tlhaloso ea Sehlahisoa

Nomoro ea Taelo: AS-1-Rb
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo

Lintho tse bonahalang	Cap	Ho tsepamisa maikutlo	Chelete	Buffer	Polokelo
Mohala oa Adapter 1 - Rb	Ntsho	400 nM	5 x 100µL	TE40 [1]	-20°C

Bakeng sa tšebeliso ea lipatlisiso feela.

• Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho label.
• Ho qoba linako tse ngata tsa ho qhibiliha ha leqhoa ka kopo, aliquot the nanolever

Tokisetso | TŠOAKA&RUTHA

In-solution hybridization ea adaptara le likhoele tsa ligand:

1.  Kopanya Adapter strand 1 - Rb (400 nM) le conjugated Ligand strand (500 nM) ka karolelano ea 1: 1 (v / v).
2.  Eketsa tharollo ea mohato oa 1 ho RT ka 600 rpm bakeng sa metsotso e 30 ho etsa bonnete ba ho kopanya ka ho feletseng.
3.  Kopanya tharollo ea mohato oa 2 le Adapter strand 1 - Rb - lfs (200 nM) ka karolelano ea 1: 1 (v / v). Tharollo e se e loketse ho sebelisoa bakeng sa ts'ebetso ea biochip. Ho tsitsa ha tharollo ho amana le botsitso ba molek'hule ea ligand

Lethathamo la 2. Lintlha tse ling bakeng sa ts'ebetso ea sebaka sa 1 le sebaka sa 2 sa litšupiso.

Lintho tse bonahalang	Ho tsepamisa maikutlo	Buffer	Related Product Name	Odara No
Ligand strand ho jara mothapo o kopaneng	500 nM	P2] E40 [	helikoptaX® Amine Coupling Kit 1	HK-NHS-1
Adapter strand 1 - Rb - lfs	200/250 nM	TE40 [1]	Adapter strand 2 e nang le dae e khubelu Rb e kopantsoeng le khoele e se nang ligand	AS-2-Rb-lfs

Example
Bophahamo bo hlokahalang bakeng sa ts'ebetso e 3: 100 μL e nang le khatello ea ho qetela ea 100 nM.

Vial 1		Vial 2
Mohala oa Adapter 1 - Rb (400 nM)	Conjugated Khoele ea ligand (500 nM)	Adapter strand 1 - Rb - lfs (200/250 nM)
25µL	25µL	50µL

Kamora nako ea ho elisa, kopanya vial 1 le vial 2 ho fumana 100 µL ea tharollo ea DNA e seng e loketse ho sebelisoa.

Ikopanye
Dynamic Biosensors GmbH

• Perchtinger Str. 8/10
• 81379 Munich
• Jeremane

Litlhaloso tsa likarolo tsa Dynamic Biosensors, Inc.

• 300 Trade Center, Suite 1400
• Woburn, MA 01801
• usa

Lintlha tsa Odara order@dynamic-biosensors.com
Tšehetso ea tekheniki support@dynamic-biosensors.com

www.dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane. ©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Litokelo tsohle li sirelelitsoe.

1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Haeba protheine e sa tsitsa ho PE40 (TE40, HE40), ka kopo, hlahloba ts'ebetso ea buffer le letlapa la ho lumellana ha switchSENSE®.

www.dynamic-biosensors.com

Litokomane / Lisebelisoa

BIOSENSORS heliX e matla hammoho le Adapter Strand 1 [pdf] Bukana ea Mosebelisi AS-1-Ra, AS-2-Ra-lfs, heliX mmoho le Adapter Strand 1, heliX plus, Adapter Strand 1, Strand 1

Litšupiso

• Bukana ea Mosebelisi