dynamic BIOSENSORS AS-2-Rb-lfs Adapter Strand 2 User Manual
ADAPTER STRAND 2
e nang le dae e khubelu Rb 'me e entsoe esale pele ka ligand strand Dynamic Biosensors GmbH & Inc.
AS-2-Rb-lfs v5.1
Likarolo tsa Bohlokoa
- Adapter strand 2 bakeng sa ts'ebetso ea heliX® Adapter Chip Spot 2.
- E lumellana le heliX ® Adapter Chip.
- E kenyeletsa Likhoele tsa Adapter bakeng sa 50 nchafatso.
- E loketse MIX&RUN sample litokisetso.
- Adapter strand 2 e na le dae e khubelu ea hydrophilic (Rb) e nang le tefiso e sa nke lehlakore.
heliX ® Adapter Chip Overview
Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA.
Sebaka sa 1
Sebaka sa 2
Tlhaloso ea Sehlahisoa
Nomoro ea odara : AS-2-Rb-lfs
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo
| Lintho tse bonahalang | Cap | Ho tsepamisa maikutlo | Chelete | Buffer | Polokelo |
| Adapter strand 2 - Rb - lfs | Bosoeu | 200/250 nM | 5 x 200µL | TE40 [1] | -20°C |
Bakeng sa tšebeliso ea lipatlisiso feela.
Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho label.
Ho qoba linako tse ngata tsa ho qhibiliha ha leqhoa ka kopo, aliquot the nanolever.
Tokisetso | TŠOAKA&RUTHA
In-solution hybridization ea adaptara le likhoele tsa ligand:
- Kopanya Adapter strand 1 - Rb (400 nM) le conjugated Ligand strand (500 nM) ka karolelano ea 1: 1 (v / v).
- Eketsa tharollo ea mohato oa 1 ho RT ka 600 rpm bakeng sa metsotso e 30 ho etsa bonnete ba ho kopanya ka ho feletseng.
- Kopanya tharollo ea mohato oa 2 le Adapter strand 2 - Rb - lfs (200 nM) ka karolelano ea 1: 1 (v / v).
Tharollo e se e loketse ho sebelisoa bakeng sa bio chip fictionalization.
Ho tsitsa ha tharollo ho amana le botsitso ba molek'hule ea ligand.
Lethathamo la 2. Boitsebiso bo eketsehileng bakeng sa tšōmo ea sebaka sa 1 le sebaka sa litšupiso 2.
| Lintho tse bonahalang | Ho tsepamisa maikutlo | Buffer | Related Product Name | Odara No |
| Adapter strand 1 - Rb | 400 nM | TE40 [1] | Adapter strand 1 ka dae e khubelu Rb | AS-1-Rb |
| Ligand strand ho jara mothapo o kopaneng | 500 nM | 2P] E40 [ | heliX® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Bolumo bo hlokahalang bakeng sa lits'ebetso tse 3: 100µL e nang le mohopolo oa ho qetela oa 100 nM.
| Vial 1 | Vial 2 | |
| Adapter strand 1 - Rb(400 nM) | Conjugated Ligand strand(500 nM) | Adapter strand 1 - Rb - lfs(200/250 nM) |
| 25µL | 25µL | 50µL |
Kamora nako ea ho elisa, kopanya vial 1 le vial 2 ho fumana 100 µL ea tharollo ea DNA e seng e loketse ho sebelisoa.
Ikopanye
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Jeremane
Litlhaloso tsa likarolo tsa Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
usa
Lintlha tsa Odara order@dynamic-biosensors.com
Tšehetso ea tekheniki support@dynamic-biosensors.com
www.dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Litokelo tsohle li sirelelitsoe.
- TE 40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
- Haeba protheine e sa tsitsa ho PE40 (TE40, HE40), ka kopo, hlahloba ts'ebetso ea buffer le letlapa la ho lumellana ha switchSENSE®.
AS-2-Rb-lfs v5.1 www.dynamic-biosensors.com
Litokomane / Lisebelisoa
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BIOSENSORS AS-2-Rb-lfs Adapter Strand 2 [pdf] Bukana ea Mosebelisi AS-2-Rb-lfs v5.1, AS-2-Rb-lfs Adapter Strand 2, AS-2-Rb-lfs, Adapter Strand 2, Strand 2 |
