dynamic BIOSENSORS heliXcyto Normalization Tharollo
Likarolo tsa Bohlokoa
- Bakeng sa ho tloaeleha ha mats'oao a fluorescent ho Spot 1 le Spot 2 ea heliX cyto chip.
- E thusa tšupiso e nepahetseng ea nako ea 'nete ea mats'oao a fluorescent a khubelu kapa a tala nakong ea litekanyo tsa RT-IC
- E lumellana le li-chips tsohle tsa heliX cyto
- Tharollo ea Normalization-Ra e na le dae e khubelu ea hydrophilic e nang le tefiso e le 'ngoe e ntle
- Tharollo ea Normalization-Ga e na le dae e tala ea hydrophilic e nang le tefiso e le 'ngoe e mpe
Tlhaloso ea Sehlahisoa
Nomoro ea odara: NOR-0
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo
| Lintho tse bonahalang | Cap | Ho tsepamisa maikutlo | Chelete | Polokelo |
| Normalization tharollo-Ra | namunu | 10 μM | 3x 100µL | -20 °C |
| Normalization tharollo-Ga | Botala | 10 μM | 3x 100µL | -20 °C |
Bakeng sa tšebeliso ea lipatlisiso feela.
Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho label.
Tokisetso
Khetha 'mala oa tharollo ea tloaelo (Ga kapa Ra) e tsamaellanang le' mala oa label ea hau ea analyte. Hlakola tharollo ea 10 µM normalization stock ho mahloriso a sebetsang ka buffer e sebetsang.
Molumo oa ho qetela o hlokahalang bakeng sa teko o ka fumanoa ho heliOS sample terei view ea tekolo.
Motsoako oa tharollo ea ho tloaeleha o lokela ho lumellana hoo e batlang e lekana le mahloriso a fluorophore sebakeng se phahameng ka ho fetisisa sa analyte se lokelang ho lekanyetsoa. Sena se ka baloa ho sebelisa equation e latelang:
: Ho tsepamisa mohopolo oa tharollo ea ho tloaeleha ka 'mala o lakatsehang
: Tšebeliso ea dae ka har'a tharollo e ngotsoeng ea analyte
: Khatiso e phahameng ka ho fetisisa ea analyte e lokelang ho lekanyetsoa
: Tekanyo ea ho ngola (karolelano ea dae ho sekaseka)
Litharollo tse hlapollotsoeng li ka bolokoa ho 2-8 ° C ho fihlela matsatsi a 7.
BOHLOKOA: U se ke ua kopanya Normalization solution-Ra le Normalization solution-Ga hammoho, ntle le haeba u ntse u theha tekanyo ea Mebala e Habeli (ho bala ho bapang ha kanale e tala le e khubelu).
Tlhokomeliso ea Kopo
Tekanyong ea RT-IC, lets'oao la fluorescent la tharollo ea ho tloaeleha le lokela ho ba ho mefuta e tšoanang e le lets'oao le phahameng ka ho fetisisa le tsoang ho analyte e tlanngoeng (data e tala).
Letšoao le felletseng la fluorescent le ipapisitse le khatello ea tharollo ea maemo a tloaelehileng le matla a ho hlasimolla a sebelisitsoeng tekanyong. Matla a ho hlasimolla a tlameha ho khethoa ho latela parameter e latelang:
a. Khatiso ea fluorophore ka tharollo ea analyte:
Khatiso ea fluorophore e ipapisitse le khatello ea analyte e sebelisitsoeng tekanyong hammoho le tekanyo ea ho ngolisoa ha mohlahlobi. Bakeng sa DOL e phahameng le likhakanyo tse phahameng tsa analyte, ho ka hlokahala ho theola matla a ho hlasimolla.
b. Letšoao le tlamang le lebelletsoeng:
Lipheo tse hlahisoang haholo seleng li ka kopanya limolek'hule tse ngata tsa analyte e ngotsoeng. Haeba ho na le liphofu tse matla haholo ho ka lebelloa pontšo e matla e tlamang. Ho qoba ho koala ha shutter, ho theola matla a ho hlasimolla ho ka nahanoa.
c. Mofuta oa chip:
Mefuta e fapaneng ea li-chip e na le bokaholimo bo fapaneng ba fluorescent. Ha maraba a le maholo le maraba a mangata ho chip, lets'oao la bokamorao le phahame. Ka hona, li-chips tsa L5 li ka hloka matla a tlase a ho hlasimolla ho feta ho sebelisoa ho li-chip tsa M5.
Bakeng sa ntlha ea ho qala ea matla a tsosang takatso le tloaelo. tharollo ea tharollo e tla sebelisoa tekong ea RT-IC, ka kopo sheba Lethathamo la 2.
Tafole 2. Relation of fluorophore concentration, normalization solution concentration, le excitation power e loketseng chip ea heliX cyto M5.
| Analyte dye conc. = analyte conc x DOL | Matla a ho hlasimolla | Concentration Normalization tharollo | Dilution Normalization tharollo |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 μM | 0.05 | 1 μM | 1:10 |
| 2.5 μM | 0.02 | 2.5 μM | 1:4 |
Hlokomela: Tafole ena ke ea tataiso ea hau. Leha ho le joalo, letšoao la ho qetela le tlalehiloeng ho heliX cyto le itšetlehile ka lintlha tse ngata. Kahoo, ho tla hlokahala ntlafatso e itseng bakeng sa sistimi ka 'ngoe.
Ikopanye
| Dynamic Biosensors GmbH | Litlhaloso tsa likarolo tsa Dynamic Biosensors, Inc. |
| Perchtinger Str. 8/10 | 300 Trade Center, Suite 1400 |
| 81379 Munich | Woburn, MA 01801 |
| Jeremane | usa |
Lintlha tsa Odara order@dynamic-biosensors.com
Tšehetso ea tekheniki support@dynamic-biosensors.com
TS'ESO MOEKETSI
www.dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Litokelo tsohle li sirelelitsoe.
Litokomane / Lisebelisoa
 |
dynamic BIOSENSORS heliXcyto Normalization Tharollo [pdf] Bukana ea Mosebelisi NOR-0, heliXcyto Normalization Tharollo, heliXcyto, Tharollo ea Normalization, Tharollo |
