dynamic BIOSENSORS Normalization Solution Software
Litlhaloso
- Lebitso la Sehlahiswa: helicity
- Mofuta: Normalization Solution (dae e khubelu)
- Bakeng sa: Litekanyo tsa RT-IC ka kanaleng e khubelu
- Moetsi: Dynamic Biosensors GmbH & Inc.
- Nomoro ea odara: NOR-Ra
- Bakeng sa Lipatlisiso Sebelisa Feela
- Shelf Life: E lekantsoe, hlahloba letsatsi la ho felloa ke nako ho label
Lintlha tse amang matla a excitation:
- Mahloriso a fluorophore ka tharollo ea analyte
- Lets'oao le tlamang le lebelletsoeng
- Mofuta oa chip
Bakeng sa tataiso e tobileng mabapi le matla a ho hlasimolla le khatello ea tharollo ea maemo a tloaelehileng, sheba Lethathamo la 2 le fanoeng bukeng ea mosebelisi. Hlokomela hore ho ka hlokahala ntlafatso e itseng ho ipapisitsoe le litsamaiso tsa motho ka mong.
FAQs
- P: Ke lokela ho boloka helicity joang?
- A: Boloka heliXcyto ho latela lintlha tsa polokelo tse fanoeng bukeng ea mosebelisi. Netefatsa hore o sheba letsatsi la ho felloa ke nako ho label le ho e sebelisa pele e felloa ke nako.
- P: Na helicity e ka sebelisoa molemong oa bongaka?
- A: Che, heliXcyto ke ea tšebeliso ea lipatlisiso feela 'me ha ea lokela ho sebelisoa bakeng sa tlhahlobo ea bongaka kapa kalafo.
Likarolo tsa Bohlokoa
- Bakeng sa ho tloaeleha ha mats'oao a fluorescent ho Spot 1 le Spot 2 ea chip ea heliXcyto
- E thusa tšupiso e nepahetseng ea nako ea nnete ea matshwao a fluorescent e khubelu nakong ea litekanyo tsa RT-IC
- E lumellana le li-chips tsohle tsa helicity
- Tharollo ea Normalization (dae e khubelu) e na le dae e khubelu ea hydrophilic e nang le tefiso e le 'ngoe e ntle.
Tlhaloso ea Sehlahisoa
- Nomoro ea odara: NOR-Ra
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo
| Lintho tse bonahalang | Cap | Ketso ea ho tsepamisa maikutlo | Chelete | Polokelo |
| Ho tloaeleha tharollo-Ra | namunu | 10 μM | 6x 100µL | -20 °C |
- Bakeng sa tšebeliso ea lipatlisiso feela.
- Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho leibole.
Tokisetso
- Sebelisa tharollo ena e tloaelehileng ea dae e khubelu bakeng sa litekanyo tsa RT-IC ka kanaleng e khubelu (e itšetlehileng ka label ea analyte).
- Fokotsa tharollo ea 10 μM ea "normalization stock" molemong oa ho sebetsa ka buffer e sebetsang.
- Motsoako oa tharollo ea ho tloaeleha o lokela ho lumellana hoo e batlang e lekana le mahloriso a fluorophore sebakeng se phahameng ka ho fetisisa sa analyte se lokelang ho lekanyetsoa.
Sena se ka baloa ho sebelisa equation e latelang:
: Ho tsepamisa mohopolo oa tharollo ea ho tloaeleha ka 'mala o lakatsehang
: Tšebeliso ea dae ka har'a tharollo e ngotsoeng ea analyte
: Khatiso e phahameng ka ho fetisisa ea analyte e lokelang ho lekanyetsoa
: Tekanyo ea ho ngola (karolelano ea dae ho sekaseka)
Litharollo tse hlapollotsoeng li ka bolokoa ho 2-8 ° C ho fihlela matsatsi a 7.
Tlhokomeliso ea Kopo
Tekanyong ea RT-IC, lets'oao la fluorescent la tharollo ea ho tloaeleha le lokela ho ba boemong bo ts'oanang le lets'oao le phahameng ka ho fetisisa le tsoang ho analyte e tlantsoeng (data e tala). Letšoao le felletseng la fluorescent le ipapisitse le khatello ea tharollo ea maemo a tloaelehileng le matla a ho hlasimolla a sebelisitsoeng tekanyong. Matla a ho hlasimolla a tlameha ho khethoa ho latela li-parameter tse latelang:
- a. Khatiso ea fluorophore ka tharollo ea analyte:
- Khatiso ea fluorophore e ipapisitse le khatello ea analyte e sebelisitsoeng tekanyong hammoho le tekanyo ea ho ngolisoa ha mohlahlobi. Bakeng sa DOL e phahameng le likhakanyo tse phahameng tsa analyte, ho ka hlokahala ho theola matla a ho hlasimolla.
- b. Letšoao le tlamang le lebelletsoeng:
- Lipheo tse hlahisoang haholo seleng li ka kopanya limolek'hule tse ngata tsa analyte e ngotsoeng. Tabeng ea lipheo tse feteletseng haholo, ho ka lebelloa pontšo e matla ea ho tlama. Ho qoba ho koala ha shutter, ho theola matla a ho hlasimolla ho ka nahanoa.
- c. Mofuta oa chip:
- Mefuta e fapaneng ea li-chip e na le maemo a fapaneng a fluorescent. Ha maraba a le maholo le maraba a mangata ho chip, lets'oao la bokamorao le phahame. Ka hona, li-chips tsa L5 li ka hloka matla a tlase a ho hlasimolla ho feta ho sebelisoa ho li-chip tsa M5.
Bakeng sa ntlha ea ho qala ea matla a tsosang takatso le tloaelo. Tharollo ea tharollo e tla sebelisoa tekong ea RT-IC, ka kopo sheba Lethathamo la 2. Lethathamo la 2. Relation of fluorophore concentration, normalization solution concentration, le excitation power e loketseng chip ea heliXcyto M5.
| Analyte dye conc. = analyte cons x DOL | Matla a ho hlasimolla | Concentration Normalization tharollo | Dilution Normalization tharollo |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 μM | 0.05 | 1 μM | 1:10 |
| 2.5 μM | 0.02 | 2.5 μM | 1:4 |
Hlokomela: Tafole ena ke ea tataiso ea hau. Leha ho le joalo, pontšo ea ho qetela e tlalehiloeng ho helicity e itšetlehile ka lintlha tse ngata. Kahoo, ho tla hlokahala ntlafatso e itseng bakeng sa sistimi ka 'ngoe.
Ikopanye
- Dynamic Biosensors GmbH
- Perchtinger Str. 8/10
- 81379 Munich
- Jeremane
- Litlhaloso tsa likarolo tsa Dynamic Biosensors, Inc.
- 300 Trade Center, Suite 1400
- Woburn, MA 01801
- usa
- Lintlha tsa Odara order@dynamic-biosensors.com.
- Tšehetso ea tekheniki support@dynamic-biosensors.com.
- www.dynamic-biosensors.com.
- Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane.
- ©2024 Dynamic Biosensors GmbH
- Dynamic Biosensors, Inc. Litokelo tsohle li sirelelitsoe.
- NOR-Ra v1.0
- www.dynamic-biosensors.com.
- Dynamic Biosensors GmbH & Inc.
- NOR-Ra v1.0
Litokomane / Lisebelisoa
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dynamic BIOSENSORS Normalization Solution Software [pdf] Bukana ea Mosebelisi NOR-Ra, Normalization Solution Software, Solution Software, Software |
