BIOSENSORS e matla HK-SXT-1 Twin-Strep Tag Capture Kit
Tlhahisoleseding ya Sehlahiswa
Litlhaloso
- Lebitso la Sehlahisoa: heliX+ TWIN-STREP-TAG CAPTURE KIT e nang le dae e khubelu Ra
- Moetsi: Dynamic Biosensors GmbH & Inc.
- Mohlala: HK-SXT-1 v6.1
- Likarolo tsa Bohlokoa:
- Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA.
- Spot 1 e sebetsa ka molek'hule ea ho hapa ha Spot 2 e sebelisoa e le tšupiso ea nako ea nnete.
- Nomoro ea Taelo: HK-SXT-1
- Bakeng sa tšebeliso ea lipatlisiso feela.
- Shelf Life: E lekanyelitsoe, likhoeli tse 3 kamora ho lokisoa ha tharollo e seng e loketse ho sebelisoa
Tlhaloso ea Sehlahisoa
HeliX+ TWIN-STREP-TAG CAPTURE KIT e nang le dae e khubelu Ra e etselitsoe ho ts'oara li-ligand tse khahlang ka holim'a metsi nakong ea ho lekanya. E lumella ho lekanya li-kinetics tse tlamang analyte kapa liphetoho tse lumellanang le ho tlama ha analyte. Kit e kenyelletsa likhoele tsa ligand le adapter tse nang le lintlha tse ikhethileng le tlhaiso-leseling ea polokelo joalo ka ha ho hlalositsoe ho Lethathamo la 1.
Tokisetso
Assay Setupo ho heliOS
- Eya ho heliOS> theha New Assay Workflow> eketsa Custom Assay> load Capture with Kinetics.
- Fetola li-parameter ho latela litlhoko tsa hau 'me u tsamaise tlhahlobo.
Litekanyetso tse sisintsoeng (mohlala, sekhahla sa phallo, nako, matla a LED, joalo-joalo) li ka har'a tlhahlobo ea heliOS.
Bakeng sa lipotso tse ling, ka kopo ikopanye le sehlopha sa tšehetso ho support@dynamic-biosensors.com
Linomoro tsa Odara tse Molemo
- Chip e nang le libaka tse 2 tsa ho lemoha: ADP-48-2-0
- Bakeng sa passivation ea holim'a chip: SOL-PAS-1-5
- Bakeng sa nchafatso ea sebaka sa chip: SOL-REG-1-5
Mokhoa oa puisano
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich Jeremane
Litlhaloso tsa likarolo tsa Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801 USA
Lintlha tsa Odara
order@dynamic-biosensors.co
Tšehetso ea tekheniki
support@dynamic-biosensors.com
Lipotso tse atisang ho botsoa (FAQ)
- P: Bophelo ba shelofo ea heliX+ TWIN-STREP- ke bofe?TAG HO TLOA KIT?
- K: Thepa e na le nako e lekanyelitsoeng ea bophelo. Ka kopo, sheba letsatsi la ho felloa ke nako leiboleng. Kamora ho lokisa tharollo e seng e loketse ho sebelisoa, nako ea ho felloa ke nako ke likhoeli tse 3.
- P: Nka tsosolosa bokaholimo ba chip joang?
- A: Ho tsosolosoa ha sefahleho ho ka etsoa ka ente ea tharollo e phahameng ea pH. Mohato ona o thusa sebaka sa chip hore se khutlele boemong ba sona ba pele.
heliX+
Bukana ea Mosebelisi
TWIN-STREP-TAG CAPTURE KIT e nang le dae e khubelu Ra
Dynamic Biosensors GmbH & Inc.
HK-SXT-1 v6.1
Likarolo tsa Bohlokoa
- Kit ena e etselitsoe ho hapa Twin-Strep-taglimolek'hule tse tsoileng matsoho tse sebelisang Strep-Tactin®XT (SXT).
- E lumellana le heliX® Adapter Chip.
- E kenyelletsa likhoele tsa Adapter le Ligand strand tse fetotsoeng ka Strep-Tactin®XT bakeng sa lintlafatso tse 20.
- Bakeng sa ts'ebetso ea Spot 1 le Spot 2.
- Likhoele tsa Adapter 1 le 2 li na le dae e khubelu ea hydrophilic (Ra) e nang le tefiso e le 'ngoe e ntle.
heliX® Adapter Chip Overview
Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA. Spot 1 e sebetsa ka molek'hule ea ho hapa ha Spot 2 e sebelisoa e le tšupiso ea nako ea nnete.
Tlhaloso ea Sehlahisoa
Nomoro ea odara: HK-SXT-1
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo
| Lintho tse bonahalang | Cap | Ho tsepamisa maikutlo | Chelete | Buffer | Polokelo |
| SXT [1] - Ligand strand | Botala | 500 nM | 2 x 100µL | TE40 [2] | 2-8°C |
| Adapter strand 1 - Ra | Ntsho | 400 nM | 2 x 100µL | TE40 [2] | -20°C |
| Adapter strand 2 - Ra - lfs | Bosoeu | 200/250 nM | 2 x 200µL | TE40 [2] | -20°C |
Bakeng sa tšebeliso ea lipatlisiso feela.
Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho label. Ka mor'a ho lokisetsa tharollo e loketseng ho sebelisoa, nako ea ho fela ke likhoeli tse 3.
Mosebetsi oa heliX® SXT - tlhahlobo ea ho hapa
- The anchor strand (ssDNA) e sa tsamaeeng ka holim'a heliX® Adapter Chip e kopantsoe le likhoele tsa DNA tse tlatsetsang tse fetotsoeng ka Strep-Tactin®XT.
- mafahla-tagged ligand ea thahasello e tšoaroa ka holimo nakong ea ho lekanya.
- Tekanyo ea kinetics e tlamang ea analyte kapa phetoho ea conformational holim'a tlamahano ea analyte.
- Ho nchafatsoa ha bokaholimo ka ente ea tharollo e phahameng ea pH. Chip surface e khutlela boemong ba pele. Mohato ona o ka lateloa ke hydrization e ncha ea ligand e ncha ka Strep-Tactin®XT.
Tokisetso
- Kopanya 100 µL SXT – Ligand strand le 100 µL Adapter strand 1 – Ra.
- Eketsa tharollo ea mohato oa 1 ho RT ka 600 rpm bakeng sa metsotso e 30 ho etsa bonnete ba ho kopanya ka ho feletseng.
- Kopanya 200 µL Adapter strand 2 – Ra – lfs to the sample ka mor'a mohato oa 2.
- Tharollo (400 µL ka kakaretso) e se e loketse ho sebelisoa bakeng sa ts'ebetso ea biochip.
- Ka kopo aliquot 'me u boloke tharollo e loketseng ho sebelisoa ho 2-8°C. Sebelisa nakong ea likhoeli tse 3.
- Thepa e na le lisebelisoa bakeng sa ho lokisa tse peli tse arohaneng tse loketseng ho sebelisa tharollo ka 400 µL ka 'ngoe.
Assay Setupo ho heliOS
- E-ea ho heliOS> theha New Assay Workflow> eketsa Custom Assay> load Capture with Kinetics> fetola litekanyo ho latela litlhoko tsa hau 'me u tsamaise tlhahlobo.
- Litekanyetso tse sisintsoeng (mohlala, sekhahla sa phallo, nako, matla a LED, joalo-joalo) li ka har'a tlhahlobo ea heliOS.
- Bakeng sa lipotso tse ling, ka kopo ikopanye le sehlopha sa tšehetso ho support@dynamic-biosensors.com
Linomoro tsa Odara tse Molemo
Lethathamo la 2. Linomoro tsa Taelo
| Lebitso la Sehlahiswa | Tlhaloso | Nomoro ea odara |
| helikoptaX® Adapter Chip | Chip e nang le libaka tse 2 tsa ho lemoha | ADP-48-2-0 |
| 10x Boikemisetso tharollo | Bakeng sa passivation ea holim'a chip | SOL-PAS-1-5 |
| Tharollo ea tsosoloso | Bakeng sa nchafatso ea sebaka sa chip | SOL-REG-1-5 |
Ikopanye
- Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Jeremane
- Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 Woburn, MA 01801 USA
- Lintlha tsa Odara order@dynamic-biosensors.com
- Tšehetso ea tekheniki support@dynamic-biosensors.com
- www.dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Litokelo tsohle li sirelelitsoe.
- Strep-Tactin®XT ke sehlahisoa sa IBA GmbH
- TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
Litokomane / Lisebelisoa
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BIOSENSORS e matla HK-SXT-1 Twin-Strep Tag Capture Kit [pdf] Bukana ea Mosebelisi v6.1, HK-SXT-1 Twin-Strep Tag Capture Kit, HK-SXT-1, Twin-Strep Tag Capture Kit, Tag Capture Kit, Capture Kit, Kit |
