BIOSENSORS heliX e matla hammoho le Adapter Strand 2

Likarolo tsa Bohlokoa

• Adapter strand 2 bakeng sa ts'ebetso ea heliX® Adapter Chip Spot 2.
• E lumellana le heliX® Adapter Chip.
• E kenyelletsa likhoele tsa Adapter bakeng sa lintlafatso tse 50.
• E loketse MIX&RUN sample litokisetso.
• Adapter strand 2 e na le dae e khubelu ea hydrophobic (Rc) e nang le tefiso e sa nke lehlakore.

heliX® Adapter Chip Overview

Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA.

Tlhaloso ea Sehlahisoa

Nomoro ea Taelo: AS-2-Rc-lfs
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo

Lintho tse bonahalang	Cap	Ho tsepamisa maikutlo	Chelete	Buffer	Polokelo
Adapter strand 2 - Rc - lfs	Bosoeu	200/250 nM	5 x 200µL	TE40 [1]	-20°C

Bakeng sa tšebeliso ea lipatlisiso feela.
Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho label.
Ho qoba linako tse ngata tsa ho qhibiliha ha leqhoa ka kopo, aliquot the nanolever.

Tokisetso | TŠOAKA&RUTHA

In-solution hybridization ea adaptara le likhoele tsa ligand:

1. Kopanya Adapter strand 1 - Rc (400 nM) le conjugated Ligand strand (500 nM) ka karolelano ea 1: 1 (v / v).
2. Eketsa tharollo ea mohato oa 1 ho RT ka 600 rpm bakeng sa metsotso e 30 ho etsa bonnete ba ho kopanya ka ho feletseng.
3. Kopanya tharollo ea mohato oa 2 le Adapter strand 2 - Rc - lfs (200 nM) ka karolelano ea 1: 1 (v / v).
   Tharollo e se e loketse ho sebelisoa bakeng sa ts'ebetso ea biochip.
   Ho tsitsa ha tharollo ho amana le botsitso ba molek'hule ea ligand.

Lethathamo la 2. Lintlha tse ling bakeng sa ts'ebetso ea sebaka sa 1 le sebaka sa 2 sa litšupiso.

Lintho tse bonahalang	Ho tsepamisa maikutlo	Buffer	Related Product Name	Odara No
Adapter strand 1 - Rc	400 nM	TE40 [1]	Adapter strand 1 ka dae e khubelu Rc	AS-1-Rc
Ligand strand ho jara mothapo o kopaneng	500 nM	2P] E40 [	heliX® Amine Coupling Kit 1	HK-NHS-1

Example
Bophahamo bo hlokahalang bakeng sa ts'ebetso e 3: 100 μL e nang le khatello ea ho qetela ea 100 nM.

Vial 1		Vial 2
Adapter strand 1 - Rc (400 nM)	Conjugated Khoele ea ligand (500 nM)	Adapter strand 1 - Rc - lfs (200/250 nM)
25µL	25µL	50µL

Kamora nako ya ho dudisa, kopanya vial 1 le vial 2 ho fumana 100 μL ya tharollo ya DNA e seng e loketse ho sebediswa.

Ikopanye

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Jeremane

Litlhaloso tsa likarolo tsa Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
usa

Lintlha tsa Odara order@dynamic-biosensors.com
Tšehetso ea tekheniki support@dynamic-biosensors.com
www.dynamic-biosensors.com

Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Litokelo tsohle li sirelelitsoe.

1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Haeba protheine e sa tsitsa ho PE40 (TE40, HE40), ka kopo, hlahloba ts'ebetso ea buffer le letlapa la ho lumellana ha switchSENSE®.

www.dynamic-biosensors.com

Litokomane / Lisebelisoa

	BIOSENSORS heliX e matla hammoho le Adapter Strand 2 [pdf] Bukana ea Mosebelisi AS-2-Rc-lfs v5.1, heliX mmoho le Adapter Strand 2, Adapter Strand 2, Strand 2
	BIOSENSORS heliX e matla hammoho le Adapter Strand 2 [pdf] Bukana ea Mosebelisi heliX hammoho le Adapter Strand 2, heliX plus, Adapter Strand 2, Strand 2

Litšupiso

• Bukana ea Mosebelisi