Dynamic Biosensors helix cyto Normalization Tharollo
Litlhaloso
- Lebitso la Sehlahiswa: heliX cyto Normalization Solution (Red Dye)
- Nomoro ea odara: NOR-R2
- Bakeng sa litekanyo tsa scIC ka kanaleng e khubelu
- Bakeng sa tšebeliso ea lipatlisiso feela
- Nako e lekantsoeng ea shelf - ka kopo, sheba letsatsi la ho felloa ke nako ho label
Likarolo tsa Bohlokoa
- Bakeng sa ho tloaeleha ha mats'oao a fluorescent ho Spot 1 le Spot 2 ea chip ea heliXcyto
- E thusa tšupiso e nepahetseng ea nako ea nnete ea matshwao a fluorescent e khubelu nakong ea litekanyo tsa scIC
- E lumellana le li-chips tsohle tsa heliXcyto
- Tharollo ea Normalization (dae e khubelu) e na le dae e khubelu ea hydrophilic e nang le tefiso e le 'ngoe e mpe.
Tlhaloso ea Sehlahisoa
- Nomoro ea odara: NOR-R2
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo
| Lintho tse bonahalang | Cap | Ho tsepamisa maikutlo | Chelete | Polokelo |
| Tharollo e tloaelehileng-R2 | Khubedu | 10 μM | 6x 100µL | -20°C |
- Bakeng sa tšebeliso ea lipatlisiso feela.
- Sehlahisoa sena se na le bophelo bo fokolang ba sethala; ka kopo, sheba letsatsi la ho felloa ke nako ho label.
- Ho qoba linako tse ngata tsa ho qhibiliha ha leqhoa, ka kopo aliquot tharollo.
Tokisetso
- Sebelisa tharollo ena e khubelu ea dae e tloaelehileng bakeng sa litekanyo tsa scIC mocheng o mofubelu (e itšetlehileng ka label ea analyte).
- Hlakola tharollo ea 10 µM normalization stock ho mahloriso a sebetsang ka buffer e sebetsang.
- Motsoako oa tharollo ea ho tloaeleha o lokela ho lumellana hoo e batlang e lekana le mahloriso a fluorophore sebakeng se phahameng ka ho fetisisa sa analyte se lokelang ho lekanyetsoa. Sena se ka baloa ho sebelisa equation e latelang:
Ho tsepamisa mohopolo oa tharollo ea ho tloaeleha ka 'mala o lakatsehang:
Motsoako oa dae ka har'a motsoako oa analyte o ngotsoeng
Khatiso e phahameng ka ho fetisisa ea analyte e lokelang ho lekanyetsoa
Tekanyo ea ho ngola (karolelano ea dae ho analyte)
Litharollo tse hlapollotsoeng li ka bolokoa ho 2-8 ° C ho fihlela matsatsi a 7.
Tlhokomeliso ea Kopo
Tekanyong ea scIC, lets'oao la fluorescent la tharollo ea ho tloaeleha le lokela ho ba boemong bo ts'oanang le lets'oao le phahameng ka ho fetisisa le tsoang ho analyte e tlamiloeng (data e tala). Letšoao le felletseng la fluorescent le ipapisitse le botsitso ba tharollo ea ho tloaeleha le matla a ho hlasimolla a sebelisitsoeng tekanyong. Matla a ho hlasimolla a tlameha ho khethoa ho latela li-parameter tse latelang:
- Fluorophore concentrations in the analyte solution
Khatiso ea fluorophore e ipapisitse le khatello ea analyte e sebelisitsoeng tekanyong hammoho le tekanyo ea ho ngolisoa ha mohlahlobi. Bakeng sa DOL e phahameng le likhakanyo tse phahameng tsa analyte, ho ka hlokahala ho theola matla a ho hlasimolla. - Lets'oao le tlamang le lebelletsoeng
Lipheo tse hlahisoang haholo seleng li ka kopanya limolek'hule tse ngata tsa analyte e ngotsoeng. Ha ho e-na le lipakane tse fetelletseng haholo, ho ka lebelloa pontšo e matla e tlamang. Ho qoba ho koala ha shutter, ho theola matla a ho hlasimolla ho ka nahanoa. - Mofuta oa chip
Mefuta e fapaneng ea li-chip e na le maemo a fapaneng a fluorescent. Ha maraba a le maholo le maraba a mangata ho chip, lets'oao la bokamorao le phahame. Ka hona, li-chips tsa L5 li ka hloka matla a tlase a ho hlasimolla ho feta ho sebelisoa ho li-chip tsa M5.
Bakeng sa qalo ea matla a hlasimoloho le normSolution mabapi le mahloriso e tla sebelisoa tekong ea scIC, ka kopo sheba Lethathamo la 2.
Letlapa la 2. Kamano ea mahloriso a fluorophore, mahloriso a tharollo e tloaelehileng, le matla a hlasimollang a loketseng 
Setšoantšo sa M5
| Analyte dye conc. = analyte conc x DOL | Matla a ho hlasimolla | Concentration Normalization tharollo | Dilution Normalization tharollo |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 μM | 0.05 | 1 μM | 1:10 |
| 2.5 μM | 0.02 | 2.5 μM | 1:4 |
Hlokomela: Tafole ena ke ea tataiso ea hau. Leha ho le joalo, letšoao la ho qetela le tlalehiloeng ho heliXcyto le itšetlehile ka lintlha tse ngata. Kahoo, ho tla hlokahala ntlafatso e itseng bakeng sa sistimi ka 'ngoe.
Ikopanye
Dynamic Biosensors GmbH
Perchtinger Str. 8/10 81379 Munich, Jeremane
Bruker Scientific LLC
40 Manning Road, Manning Park, Billerica, MA 01821 USA
- Lintlha tsa Odara
- Tšehetso ea tekheniki
www.dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane. ©2025 Dynamic Biosensors GmbH Bakeng sa Tšebeliso ea Lipatlisiso Feela. Ha e sebelisoe lits'ebetsong tsa tlhahlobo ea bongaka.
FAQs
Bophelo ba shelofo ea HeliX cyto Normalization Solution ke bofe?
Sehlahisoa se na le nako e fokolang ea bophelo. Ka kopo, sheba letsatsi la ho felloa ke nako leiboleng bakeng sa lintlha tse khethehileng.
Ke lokela ho boloka sehlahisoa sa NOR-R2 v1.0 joang?
Sehlahisoa se lokela ho bolokoa ho latela tlhaiso-leseling ea polokelo e fanoeng ho Lethathamo la 1 la bukana ea mosebelisi.
A na nka sebelisa sehlahisoa see molemong oa bongaka?
Che, sehlahisoa sena ke sa tšebeliso ea lipatlisiso feela.
Litokomane / Lisebelisoa
 |
Dynamic Biosensors helix cyto Normalization Tharollo [pdf] Bukana ea Mosebelisi helix cyto Normalization Tharollo, helix cyto, Normalization Tharollo, Tharollo |