Dynamic Biosensors helix cyto Normalization Solution
Litlhaloso
- Lebitso la Sehlahiswa: heliX cyto Normalization Solution (Red Dye)
- Nomoro ea odara: NOR-R2
- For scIC measurements in the red channel
- Bakeng sa tšebeliso ea lipatlisiso feela
- Limited shelf life – please check expiry date on label
Likarolo tsa Bohlokoa
- Bakeng sa ho tloaeleha ha mats'oao a fluorescent ho Spot 1 le Spot 2 ea chip ea heliXcyto
- Enables correct real-time referencing of the red fluorescent signals during scIC measurements
- Compatible with all heliXcyto chips
- The Normalization solution (red dye) contains a hydrophilic red dye with a single negative net charge.
Tlhaloso ea Sehlahisoa
- Nomoro ea odara: NOR-R2
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo
| Lintho tse bonahalang | Cap | Concentr ation | Chelete | Polokelo |
| Normalization solution-R2 | Khubedu | 10 μM | 6x 100µL | -20°C |
- Bakeng sa tšebeliso ea lipatlisiso feela.
- This product has a limited shelf life; please see the expiry date on the label.
- To avoid many freeze-thaw cycles, please aliquot the solution.
Tokisetso
- Use this red dye normalization solution for scIC measurements in the red channel (analyte label-dependent).
- Dilute the 10 µM normalization stock solution to a working concentration with running buffer.
- The concentration of the normalization solution should approximately correspond to the fluorophore concentration in the highest analyte concentration to be measured. This can be calculated usingthe following equation:
Concentration of the normalization solution in the desired color :
Motsoako oa dae ka har'a motsoako oa analyte o ngotsoeng
Highest concentration of the analyte that should be measured
Degree of labeling (ratio of dye to analyte)
Litharollo tse hlapollotsoeng li ka bolokoa ho 2-8 ° C ho fihlela matsatsi a 7.
Tlhokomeliso ea Kopo
In the scIC measurement, the fluorescent signal of the normalization solution should be in a similar range to the highest signal coming from the bound analyte (raw data). The absolute fluorescent signal is dependent on normalization solution concentration and the excitation power applied in the measurement. The excitation power has to be selected based on the following parameters:
- Fluorophore concentration inthe analyte solution
Khatiso ea fluorophore e ipapisitse le khatello ea analyte e sebelisitsoeng tekanyong hammoho le tekanyo ea ho ngolisoa ha mohlahlobi. Bakeng sa DOL e phahameng le likhakanyo tse phahameng tsa analyte, ho ka hlokahala ho theola matla a ho hlasimolla. - Lets'oao le tlamang le lebelletsoeng
Highly expressed targets on a cell can bind more molecules of the labeled analyte. In case of highly overexpressed targets, a strong binding signal can be expected. To avoid the shutter closing, lowering the excitation power might be considered. - Mofuta oa chip
Mefuta e fapaneng ea li-chip e na le maemo a fapaneng a fluorescent. Ha maraba a le maholo le maraba a mangata ho chip, lets'oao la bokamorao le phahame. Ka hona, li-chips tsa L5 li ka hloka matla a tlase a ho hlasimolla ho feta ho sebelisoa ho li-chip tsa M5.
For a starting point of excitation power and normSolution on concentration to be used in an scIC experiment, please refer to Table 2.
Table 2. Relation of fluorophore concentration, normalization solution concentration, and excitation power suitable for an 
Setšoantšo sa M5
| Analyte dye conc. = analyte conc x DOL | Matla a ho hlasimolla | Concentration Normalization tharollo | Dilution Normalization tharollo |
| 25 nM | 0.5 | 25 nM | 1:400 |
| 50 nM | 0.3 | 50 nM | 1:200 |
| 100 nM | 0.2 | 100 nM | 1:100 |
| 300 nM | 0.1 | 300 nM | 1:33 |
| 500 nM | 0.08 | 500 nM | 1:20 |
| 1 μM | 0.05 | 1 μM | 1:10 |
| 2.5 μM | 0.02 | 2.5 μM | 1:4 |
Hlokomela: This table is for your guidance. However, the final signal recorded in the heliXcyto depends on many factors. Thus, some optimization will be required for each system.
Ikopanye
Dynamic Biosensors GmbH
Perchtinger Str. 8/10 81379 Munich, Germany
Bruker Scientific LLC
40 Manning Road, Manning Park, Billerica, MA 01821 USA
- Lintlha tsa Odara
- Tšehetso ea tekheniki
www.dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane. ©2025 Dynamic Biosensors GmbH Bakeng sa Tšebeliso ea Lipatlisiso Feela. Ha e sebelisoe lits'ebetsong tsa tlhahlobo ea bongaka.
FAQs
What is the shelf life of the heliX cyto Normalization Solution?
Sehlahisoa se na le nako e fokolang ea bophelo. Ka kopo, sheba letsatsi la ho felloa ke nako leiboleng bakeng sa lintlha tse khethehileng.
How should I store the NOR-R2 v1.0 product?
The product should be stored according to the storage information provided in Table 1 of the user manual.
Can I use this product for clinical purposes?
No, this product is for research use only.
Litokomane / Lisebelisoa
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Dynamic Biosensors helix cyto Normalization Solution [pdf] Bukana ea Mosebelisi helix cyto Normalization Solution, helix cyto, Normalization Solution, Solution |