Dynamic Biosensors Adapter Strand Package
Likarolo tsa Bohlokoa
- Adapter strand 1 - Ga le Adapter strand 2 - Ga - lfs (ligand free strand) bakeng sa ts'ebetso ea heliX ® Adapter Chip ho Spot 1 le Spot 2, ka ho latellana.
- E lumellana le heliX ® Adapter Chip.
- E kenyelletsa likhoele tsa Adapter bakeng sa lintlafatso tse 50.
- E loketse MIX&RUN sample litokisetso.
- Likhoele tsa Adapter 1 le 2 li na le dae e tala ea hydrophilic (Ga) e nang le tefiso e le 'ngoe e mpe.
Adapter Chip Overview
Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA.
Tlhaloso ea Sehlahisoa
Nomoro ea odara: ASP-1-Ga
Lethathamo la 1. Litaba le Boitsebiso ba Polokelo
| Lintho tse bonahalang | Cap | Ho tsepamisa maikutlo | Chelete | Buffer | Polokelo |
| Adapter strand 1 - Ga | Ntsho | 400 nM | 5 x 100µL | TE40 [1] | -20°C |
| Adapter strand 2 - Ga - lfs | Bosoeu | 200/250 nM | 5 x 200µL | TE40 [1] | -20°C |
Bakeng sa tšebeliso ea lipatlisiso feela.
Sehlahisoa sa hae se na le nako ea bophelo bo fokolang, ka kopo, sheba letsatsi la ho felloa ke nako ho label.
Ho qoba linako tse ngata tsa ho qhibiliha ha leqhoa ka kopo, aliquot the nano lever.
Tokisetso | Kopanya&matha
In-solution hybridization ea adaptara le likhoele tsa ligand:
- Kopanya Adapter strand 1 - Ga (400 nM) le conjugated Ligand strand (500 nM) ka karolelano ea 1: 1 (v / v).
- Eketsa tharollo ea mohato oa 1 ho RT ka 600 rpm bakeng sa metsotso e 30 ho etsa bonnete ba ho kopanya ka ho feletseng.
- Kopanya tharollo ea mohato oa 2 le Adapter strand 2 - Ga - lfs (200 nM) ka karolelano ea 1: 1 (v / v).
Tharollo e se e loketse ho sebelisoa bakeng sa ts'ebetso ea biochip.
Ho tsitsa ha tharollo ho amana le botsitso ba molek'hule ea ligand.
Lethathamo la 2. Boitsebiso bo eketsehileng bakeng sa ts'ebetso ea sebaka sa 1 le sebaka sa boitsebiso 2.
| Lintho tse bonahalang | Ho tsepamisa maikutlo | Buffer | Related Product Name | Odara No |
| Ligand strand e jereng ligand e kopaneng | 500 nM | PE40 [2] | heliX ® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Bophahamo bo hlokahalang bakeng sa lits'ebetso tse 3: 100 µL e nang le khatello ea ho qetela ea 100 nM.
| Vial 1 | Vial 2 | |
| Adapter strand 1 – Ga (400 nM) | Khoele e kopaneng ea Ligand (500 nM) | Adapter strand 2 – Ga – lfs (200/250 nM) |
| 25µL | 25µL | 50µL |
Kamora nako ea ho elisa, kopanya vial 1 le vial 2 ho fumana 100 µL ea tharollo ea DNA e seng e loketse ho sebelisoa.
Tšehetso ea Bareki
Ikopanye
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Jeremane
Bruker Scientific LLC
40 Manning Road, Manning Park
Billerica, MA 01821
usa
Lintlha tsa Odara order@dynamic-biosensors.com
Tšehetso ea tekheniki support@dynamic-biosensors.com
www.dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane.
©2025 Dynamic Biosensors GmbH
Bakeng sa Tšebeliso ea Lipatlisiso Feela. Ha e sebelisoe lits'ebetsong tsa tlhahlobo ea bongaka.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
[2] Haeba protheine e sa tsitsa ho PE40 (TE40, HE40), ka kopo, hlahloba hore buffer e lumellana le switchSENSE®.
letlapa la tumellano.
Litokomane / Lisebelisoa
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Dynamic Biosensors Adapter Strand Package [pdf] Bukana ea Mosebelisi Adapter Strand Package, Strand Package, Package |