Dynamic Biosensors HK-GFP-1 GFP Capture Kit Bukana ea Mosebelisi

HK-GFP-1 GFP Capture Kit

“

Litlhaloso:

• Lebitso la Sehlahisoa: heliX + GFP CAPTURE KIT e nang le dae e khubelu Ra
• Moetsi: Dynamic Biosensors GmbH
• Mohlala: HK-GFP-1 v3.1
• Likarolo tsa Bohlokoa:
  • Matheba a 2 a nang le tatelano e 2 e fapaneng ea ankora bakeng sa DNA-encoded
    ho bua
  • Sebaka sa 1 se sebetsa ka molek'hule ea ho hapa
  • Spot 2 e sebelisoa e le litšupiso tsa nako ea nnete

Tlhaloso ea Sehlahisoa:

Nomoro ea Taelo: HK-GFP-1

Litaba le Boitsebiso ba Polokelo:

Lintho tse bonahalang	Ho tsepamisa maikutlo
GFP e tlamang protheine - Ligand strand Green	500 nM
Adapter strand 1 - Ra Black	400 nM
Adapter strand 2 - Ra - lfs White	200/250 nM

Litaelo tsa Tšebeliso ea Sehlahisoa:

Mokhoa oa ho lekanya:

1. GFP-tagged ligand e tšoaroa ka holim'a metsi nakong ea
   ho lekanya matha.
2. Lekanya kinetics e tlamang ea analyte kapa phetoho ea conformational
   holim'a ho tlama ha analyte.
3. Hlahisa bokaholimo ka ho kenya tharollo e phahameng ea pH ho
   e busetse boemong ba pele.

Boitokisetso:

Sethapo sa Assay ho heliOS:

1. Eya ho heliOS> theha New Assay Workflow > eketsa Custom
Assay> laela Kinetics ka Capture> fetola litekanyo tse thehiloeng
ka litlhoko tsa hau mme u tsamaise tlhahlobo.

Litekanyetso tse khothalelitsoeng:

Sekhahla sa phallo, nako, matla a LED, joalo-joalo, li ka har'a tlhahlobo ea heliOS.
Bakeng sa lintlha tse ling, ikopanye le support@dynamic-biosensors.com.

LBH:

Linomoro tsa Odara tse Molemo:

• Chip e nang le libaka tse 2 tsa ho lemoha: Order No ADP-48-2-0
• Passivation ea sefahleho sa chip: Order No SOL-PAS-1-5
• Ho tsosolosoa ha sebaka sa chip: Order No SOL-REG-1-5

Mokhoa oa puisano:

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich Jeremane

Bruker Scientific LLC
40 Manning Road, Mamming Park Billerica, MA 01821 USA

Lintlha tsa Odara:
order@dynamic-biosensors.com
Tšehetso ea tekheniki:
support@dynamic-biosensors.com

Hlokomela: Bakeng sa lintlha tse qaqileng tsa tekheniki le
lintlha tse eketsehileng, sheba bukana ea molao ea lihlahisoa ho
www.dynamic-biosensors.com.

"`

heliX +
Bukana ea Mosebelisi
GFP CAPTURE KIT
ka dae e khubelu Ra
Dynamic Biosensors GmbH HK-GFP-1 v3.1

Likarolo tsa Bohlokoa
· Setsi sena se etselitsoe ho hapa GFP-tagged ligands e sebelisang protheine e tlamang ea GFP. · E lumellana le heliX® Adapter Chip. * E kenyelletsa likhoele tsa Adapter le Ligand strand tse fetotsoeng ka protheine e tlamang ea GFP bakeng sa lintlafatso tse 20. · Bakeng sa ts'ebetso ea Spot 1 le Spot 2. · Likhoele tsa Adapter 1 le 2 li na le dae e khubelu ea hydrophilic (Ra) e nang le tefiso e le 'ngoe e ntle.
heliX® Adapter Chip Overview
Libaka tse 2 tse nang le tatellano e 2 e fapaneng ea li-ankora bakeng sa aterese e kentsoeng ka DNA. Spot 1 e sebetsa ka molek'hule ea ho hapa ha Spot 2 e sebelisoa e le tšupiso ea nako ea nnete.

Tlhaloso ea Sehlahisoa

Nomoro ea Taelo: HK-GFP-1

Lethathamo la 1. Litaba le Boitsebiso ba Polokelo

Lintho tse bonahalang

Cap

GFP e tlamang protheine [1] - Ligand strand Green

Adapter strand 1 - Ra

Ntsho

Adapter strand 2 - Ra - lfs

Bosoeu

Tekanyo ea 500 nM 400 nM 200/250 nM

Chelete 2 x 100 µL 2 x 100 µL 2 x 200 µL

Bakeng sa tšebeliso ea lipatlisiso feela.
Sehlahisoa sena se na le nako ea nako ea shelofo, ka kopo, sheba letsatsi la ho felloa ke nako ho label. Ka mor'a ho lokisetsa tharollo e loketseng ho sebelisoa, nako ea ho fela ke likhoeli tse 3.

Buffer TE40 [2] TE40 [2] TE40 [2]

Ho boloka 2-8°C -20°C -20°C

HK-GFP-1 v3.1

www.dynamic-biosensors.com

Leqephe la 1 la 4

Mosebetsi oa heliX® GFP - tlhahlobo ea ho hapa

1. The anchor strand (ssDNA) e sa tsamaeeng ka holim'a heliX® Adapter Chip e kopantsoe le likhoele tsa DNA tse tlatsetsang tse fetotsoeng ka protheine e tlamang ea GFP.
2. GFP-tagged ligand ea thahasello e tšoaroa ka holimo nakong ea ho lekanya. 3. Tekanyo ea kinetics e tlamang ea analyte kapa phetoho ea conformational holim'a tlamahano ea analyte. 4. Ho tsosolosoa ha sefahleho ka ente ea tharollo e phahameng ea pH. Chip surface e khutlela boemong ba pele. Mohato ona e ka ba
e lateloe ke hydrization e ncha ea ligand e ncha e nang le protheine e tlamang ea GFP.
Tokisetso
1. Kopanya 100 µL GFP e tlamang protheine - Ligand strand e nang le 100 µL Adapter strand 1 - Ra. 2. Eketsa tharollo ea mohato oa 1 ho RT ho 600 rpm bakeng sa 30 min ho etsa bonnete ba ho kopanya ka ho feletseng. 3. Kopanya 200 µL Adapter strand 2 – Ra – lfs to the sample ka mor'a mohato oa 2.
Tharollo (400 µL ka kakaretso) e se e loketse ho sebelisoa bakeng sa ts'ebetso ea biochip.
Ka kopo aliquot 'me u boloke tharollo e loketseng ho sebelisoa ho 2-8°C. Sebelisa nakong ea likhoeli tse 3.
Thepa e na le lisebelisoa bakeng sa ho lokisa tse peli tse arohaneng tse loketseng ho sebelisa tharollo ka 400 µL ka 'ngoe.
Assay Setupo ho heliOS
E-ea ho heliOS> theha New Assay Workflow> eketsa Custom Assay> mojaro Kinetics ka Capture> fetola litekanyetso ho latela litlhoko tsa hau mme u tsamaise tlhahlobo.
Litekanyetso tse sisintsoeng (mohlala, sekhahla sa phallo, nako, matla a LED, joalo-joalo) li ka har'a tlhahlobo ea heliOS.
Bakeng sa lipotso tse ling, ka kopo ikopanye le sehlopha sa tšehetso ho support@dynamic-biosensors.com

HK-GFP-1 v3.1

www.dynamic-biosensors.com

Leqephe la 2 la 4

Linomoro tsa Odara tse Molemo
Letlapa la 2. Linomoro tsa Linomoro Lebitso la Sehlahisoa heliX® Adapter Chip 10x Passivation solution Tharollo ea ho tsosolosa

Comment Chip e nang le matheba a 2 a ho lemoha Bakeng sa passivation ea chip surface Bakeng sa nchafatso ea holim'a chip

Odara No ADP-48-2-0 SOL-PAS-1-5 SOL-REG-1-5

HK-GFP-1 v3.1

www.dynamic-biosensors.com

Leqephe la 3 la 4

Ikopanye

Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Jeremane

Bruker Scientific LLC 40 Manning Road, Mamming Park Billerica, MA 01821 USA

Odara Tlhahisoleseding order@dynamic-biosensors.com Tšehetso ea Tekheniki support@dynamic-biosensors.com
www.dynamic-biosensors.com
Lisebelisoa le li-chips li entsoe le ho etsoa Jeremane. ©2025 Dynamic Biosensors GmbH Bakeng sa Tšebeliso ea Lipatlisiso Feela. Ha e sebelisoe lits'ebetsong tsa tlhahlobo ea bongaka.

[1] GFP e tlamang protheine ke sehlahisoa sa ChromoTek GmbH [2] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 M EDTA, 50 M EGTA

HK-GFP-1 v3.1

www.dynamic-biosensors.com

Leqephe la 4 la 4

Litokomane / Lisebelisoa

Dynamic Biosensors HK-GFP-1 GFP Capture Kit [pdf] Bukana ea Mosebelisi HK-GFP-1 GFP Capture Kit, HK-GFP-1, GFP Capture Kit, Capture Kit

Litšupiso

• Bukana ea Mosebelisi